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Rodney Kim Tweten

TitleGeorge L Cross Research Professor
InstitutionUniversity of Oklahoma Health Sciences Center
DepartmentMicrobiology & Immunology
Address975 NE 10th St
Oklahoma City OK 73104-5419
Phone405/271-1205
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    Collapse Research 
    Collapse research activities and funding
    R01AI026895     (TWETEN, RODNEY K.)Jul 1, 1988 - Jun 30, 1992
    NIH/NIAID
    STUDIES ON CLOSTRIDIUM DIFFICILE TOXIN B
    Role: Principal Investigator

    R01AI032097     (TWETEN, RODNEY K.)Jan 1, 1993 - Jun 30, 2005
    NIH/NIAID
    STUDIES ON CLOSTRIDIUM SEPTICUM LETHAL TOXIN
    Role: Principal Investigator

    R01AI037657     (TWETEN, RODNEY K.)Apr 1, 1997 - Mar 31, 2021
    NIH/NIAID
    Pore Formation by Cholesterol Dependent Cytolysins
    Role: Principal Investigator

    R01AI063444     (TWETEN, RODNEY K.)Jan 15, 2005 - Dec 31, 2009
    NIH/NIAID
    A Novel Cholesterol-Dependent Cytolysin Receptor
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Lawrence SL, Gorman MA, Feil SC, Mulhern TD, Kuiper MJ, Ratner AJ, Tweten RK, Morton CJ, Parker MW. Structural Basis for Receptor Recognition by the Human CD59-Responsive Cholesterol-Dependent Cytolysins. Structure. 2016 Sep 06; 24(9):1488-98. PMID: 27499440.
      View in: PubMed
    2. Ellisdon AM, Reboul CF, Panjikar S, Huynh K, Oellig CA, Winter KL, Dunstone MA, Hodgson WC, Seymour J, Dearden PK, Tweten RK, Whisstock JC, McGowan S. Stonefish toxin defines an ancient branch of the perforin-like superfamily. Proc Natl Acad Sci U S A. 2015 Dec 15; 112(50):15360-5. PMID: 26627714; PMCID: PMC4687532.
    3. Lawrence SL, Feil SC, Morton CJ, Farrand AJ, Mulhern TD, Gorman MA, Wade KR, Tweten RK, Parker MW. Crystal structure of Streptococcus pneumoniae pneumolysin provides key insights into early steps of pore formation. Sci Rep. 2015 Sep 25; 5:14352. PMID: 26403197; PMCID: PMC4585913.
    4. Chen A, Mann B, Gao G, Heath R, King J, Maissoneuve J, Alderson M, Tate A, Hollingshead SK, Tweten RK, Briles DE, Tuomanen EI, Paton JC. Multivalent Pneumococcal Protein Vaccines Comprising Pneumolysoid with Epitopes/Fragments of CbpA and/or PspA Elicit Strong and Broad Protection. Clin Vaccine Immunol. 2015 Oct; 22(10):1079-89. PMID: 26245351; PMCID: PMC4580740.
    5. Farrand AJ, Hotze EM, Sato TK, Wade KR, Wimley WC, Johnson AE, Tweten RK. The Cholesterol-dependent Cytolysin Membrane-binding Interface Discriminates Lipid Environments of Cholesterol to Support ß-Barrel Pore Insertion. J Biol Chem. 2015 Jul 17; 290(29):17733-44. PMID: 26032415; PMCID: PMC4505022.
    6. Wade KR, Tweten RK. The Apicomplexan CDC/MACPF-like pore-forming proteins. Curr Opin Microbiol. 2015 Aug; 26:48-52. PMID: 26025132; PMCID: PMC4577444.
    7. Verherstraeten S, Goossens E, Valgaeren B, Pardon B, Timbermont L, Haesebrouck F, Ducatelle R, Deprez P, Wade KR, Tweten R, Van Immerseel F. Perfringolysin O: The Underrated Clostridium perfringens Toxin? Toxins (Basel). 2015 May 14; 7(5):1702-21. PMID: 26008232; PMCID: PMC4448169.
    8. Lukoyanova N, Kondos SC, Farabella I, Law RH, Reboul CF, Caradoc-Davies TT, Spicer BA, Kleifeld O, Traore DA, Ekkel SM, Voskoboinik I, Trapani JA, Hatfaludi T, Oliver K, Hotze EM, Tweten RK, Whisstock JC, Topf M, Saibil HR, Dunstone MA. Conformational changes during pore formation by the perforin-related protein pleurotolysin. PLoS Biol. 2015 Feb; 13(2):e1002049. PMID: 25654333; PMCID: PMC4318580.
    9. Wade KR, Hotze EM, Kuiper MJ, Morton CJ, Parker MW, Tweten RK. An intermolecular electrostatic interaction controls the prepore-to-pore transition in a cholesterol-dependent cytolysin. Proc Natl Acad Sci U S A. 2015 Feb 17; 112(7):2204-9. PMID: 25646411; PMCID: PMC4343107.
    10. Bolz DD, Li Z, McIndoo ER, Tweten RK, Bryant AE, Stevens DL. Cardiac myocyte dysfunction induced by streptolysin O is membrane pore and calcium dependent. Shock. 2015 Feb; 43(2):178-84. PMID: 25243426; PMCID: PMC4297253.
    11. Tweten RK, Hotze EM, Wade KR. The Unique Molecular Choreography of Giant Pore Formation by the Cholesterol-Dependent Cytolysins of Gram-Positive Bacteria. Annu Rev Microbiol. 2015; 69:323-40. PMID: 26488276.
      View in: PubMed
    12. Wade KR, Hotze EM, Briles DE, Tweten RK. Mouse, but not human, ApoB-100 lipoprotein cholesterol is a potent innate inhibitor of Streptococcus pneumoniae pneumolysin. PLoS Pathog. 2014 Sep; 10(9):e1004353. PMID: 25188225; PMCID: PMC4154877.
    13. Harvey RM, Hughes CE, Paton AW, Trappetti C, Tweten RK, Paton JC. The impact of pneumolysin on the macrophage response to Streptococcus pneumoniae is strain-dependent. PLoS One. 2014; 9(8):e103625. PMID: 25105894; PMCID: PMC4126675.
    14. Feil SC, Ascher DB, Kuiper MJ, Tweten RK, Parker MW. Structural studies of Streptococcus pyogenes streptolysin O provide insights into the early steps of membrane penetration. J Mol Biol. 2014 Feb 20; 426(4):785-92. PMID: 24316049; PMCID: PMC4323271.
    15. Mann B, Thornton J, Heath R, Wade KR, Tweten RK, Gao G, El Kasmi K, Jordan JB, Mitrea DM, Kriwacki R, Maisonneuve J, Alderson M, Tuomanen EI. Broadly protective protein-based pneumococcal vaccine composed of pneumolysin toxoid-CbpA peptide recombinant fusion protein. J Infect Dis. 2014 Apr 01; 209(7):1116-25. PMID: 24041791; PMCID: PMC3952665.
    16. Sato TK, Tweten RK, Johnson AE. Disulfide-bond scanning reveals assembly state and ß-strand tilt angle of the PFO ß-barrel. Nat Chem Biol. 2013 Jun; 9(6):383-9. PMID: 23563525; PMCID: PMC3661704.
    17. Lawrence SL, Feil SC, Holien JK, Kuiper MJ, Doughty L, Dolezal O, Mulhern TD, Tweten RK, Parker MW. Manipulating the Lewis antigen specificity of the cholesterol-dependent cytolysin lectinolysin. Front Immunol. 2012; 3:330. PMID: 23181061; PMCID: PMC3500998.
    18. Hotze EM, Le HM, Sieber JR, Bruxvoort C, McInerney MJ, Tweten RK. Identification and characterization of the first cholesterol-dependent cytolysins from Gram-negative bacteria. Infect Immun. 2013 Jan; 81(1):216-25. PMID: 23115036; PMCID: PMC3536126.
    19. Dowd KJ, Farrand AJ, Tweten RK. The cholesterol-dependent cytolysin signature motif: a critical element in the allosteric pathway that couples membrane binding to pore assembly. PLoS Pathog. 2012; 8(7):e1002787. PMID: 22792065; PMCID: PMC3390400.
    20. Dunstone MA, Tweten RK. Packing a punch: the mechanism of pore formation by cholesterol dependent cytolysins and membrane attack complex/perforin-like proteins. Curr Opin Struct Biol. 2012 Jun; 22(3):342-9. PMID: 22658510; PMCID: PMC3383384.
    21. Hotze EM, Wilson-Kubalek E, Farrand AJ, Bentsen L, Parker MW, Johnson AE, Tweten RK. Monomer-monomer interactions propagate structural transitions necessary for pore formation by the cholesterol-dependent cytolysins. J Biol Chem. 2012 Jul 13; 287(29):24534-43. PMID: 22645132; PMCID: PMC3397878.
    22. Feil SC, Lawrence S, Mulhern TD, Holien JK, Hotze EM, Farrand S, Tweten RK, Parker MW. Structure of the lectin regulatory domain of the cholesterol-dependent cytolysin lectinolysin reveals the basis for its lewis antigen specificity. Structure. 2012 Feb 08; 20(2):248-58. PMID: 22325774; PMCID: PMC3682648.
    23. Vadia S, Arnett E, Haghighat AC, Wilson-Kubalek EM, Tweten RK, Seveau S. The pore-forming toxin listeriolysin O mediates a novel entry pathway of L. monocytogenes into human hepatocytes. PLoS Pathog. 2011 Nov; 7(11):e1002356. PMID: 22072970; PMCID: PMC3207921.
    24. Lencer WI, Tweten RK. To translocate or not: that is the problem. Cell Host Microbe. 2011 Sep 15; 10(3):179-80. PMID: 21925105; PMCID: PMC3178040.
    25. Hotze EM, Tweten RK. Membrane assembly of the cholesterol-dependent cytolysin pore complex. Biochim Biophys Acta. 2012 Apr; 1818(4):1028-38. PMID: 21835159; PMCID: PMC3243806.
    26. Wickham SE, Hotze EM, Farrand AJ, Polekhina G, Nero TL, Tomlinson S, Parker MW, Tweten RK. Mapping the intermedilysin-human CD59 receptor interface reveals a deep correspondence with the binding site on CD59 for complement binding proteins C8alpha and C9. J Biol Chem. 2011 Jun 10; 286(23):20952-62. PMID: 21507937; PMCID: PMC3121471.
    27. Farrand AJ, LaChapelle S, Hotze EM, Johnson AE, Tweten RK. Only two amino acids are essential for cytolytic toxin recognition of cholesterol at the membrane surface. Proc Natl Acad Sci U S A. 2010 Mar 02; 107(9):4341-6. PMID: 20145114; PMCID: PMC2840085.
    28. Flanagan JJ, Tweten RK, Johnson AE, Heuck AP. Cholesterol exposure at the membrane surface is necessary and sufficient to trigger perfringolysin O binding. Biochemistry. 2009 May 12; 48(18):3977-87. PMID: 19292457; PMCID: PMC2825173.
    29. LaChapelle S, Tweten RK, Hotze EM. Intermedilysin-receptor interactions during assembly of the pore complex: assembly intermediates increase host cell susceptibility to complement-mediated lysis. J Biol Chem. 2009 May 08; 284(19):12719-26. PMID: 19293153; PMCID: PMC2676001.
    30. Kennedy CL, Lyras D, Cordner LM, Melton-Witt J, Emmins JJ, Tweten RK, Rood JI. Pore-forming activity of alpha-toxin is essential for clostridium septicum-mediated myonecrosis. Infect Immun. 2009 Mar; 77(3):943-51. PMID: 19139192; PMCID: PMC2643643.
    31. Farrand S, Hotze E, Friese P, Hollingshead SK, Smith DF, Cummings RD, Dale GL, Tweten RK. Characterization of a streptococcal cholesterol-dependent cytolysin with a lewis y and b specific lectin domain. Biochemistry. 2008 Jul 08; 47(27):7097-107. PMID: 18553932; PMCID: PMC2622431.
    32. Miersch S, Espey MG, Chaube R, Akarca A, Tweten R, Ananvoranich S, Mutus B. Plasma membrane cholesterol content affects nitric oxide diffusion dynamics and signaling. J Biol Chem. 2008 Jul 04; 283(27):18513-21. PMID: 18445594; PMCID: PMC2441544.
    33. Hu W, Ferris SP, Tweten RK, Wu G, Radaeva S, Gao B, Bronson RT, Halperin JA, Qin X. Rapid conditional targeted ablation of cells expressing human CD59 in transgenic mice by intermedilysin. Nat Med. 2008 Jan; 14(1):98-103. PMID: 18157141.
      View in: PubMed
    34. Soltani CE, Hotze EM, Johnson AE, Tweten RK. Structural elements of the cholesterol-dependent cytolysins that are responsible for their cholesterol-sensitive membrane interactions. Proc Natl Acad Sci U S A. 2007 Dec 18; 104(51):20226-31. PMID: 18077338; PMCID: PMC2154413.
    35. Soltani CE, Hotze EM, Johnson AE, Tweten RK. Specific protein-membrane contacts are required for prepore and pore assembly by a cholesterol-dependent cytolysin. J Biol Chem. 2007 May 25; 282(21):15709-16. PMID: 17412689; PMCID: PMC3746338.
    36. Rossjohn J, Polekhina G, Feil SC, Morton CJ, Tweten RK, Parker MW. Structures of perfringolysin O suggest a pathway for activation of cholesterol-dependent cytolysins. J Mol Biol. 2007 Apr 13; 367(5):1227-36. PMID: 17328912; PMCID: PMC3674820.
    37. Melton-Witt JA, Bentsen LM, Tweten RK. Identification of functional domains of Clostridium septicum alpha toxin. Biochemistry. 2006 Dec 05; 45(48):14347-54. PMID: 17128973; PMCID: PMC2561313.
    38. Salles II, Voth DE, Ward SC, Averette KM, Tweten RK, Bradley KA, Ballard JD. Cytotoxic activity of Bacillus anthracis protective antigen observed in a macrophage cell line overexpressing ANTXR1. Cell Microbiol. 2006 Aug; 8(8):1272-81. PMID: 16882031.
      View in: PubMed
    39. Tweten RK. Cholesterol-dependent cytolysins, a family of versatile pore-forming toxins. Infect Immun. 2005 Oct; 73(10):6199-209. PMID: 16177291; PMCID: PMC1230961.
    40. Dang TX, Milligan RA, Tweten RK, Wilson-Kubalek EM. Helical crystallization on nickel-lipid nanotubes: perfringolysin O as a model protein. J Struct Biol. 2005 Nov; 152(2):129-39. PMID: 16242343.
      View in: PubMed
    41. Schuerch DW, Wilson-Kubalek EM, Tweten RK. Molecular basis of listeriolysin O pH dependence. Proc Natl Acad Sci U S A. 2005 Aug 30; 102(35):12537-42. PMID: 16105950; PMCID: PMC1194900.
    42. Ramachandran R, Tweten RK, Johnson AE. The domains of a cholesterol-dependent cytolysin undergo a major FRET-detected rearrangement during pore formation. Proc Natl Acad Sci U S A. 2005 May 17; 102(20):7139-44. PMID: 15878993; PMCID: PMC1129106.
    43. Dang TX, Hotze EM, Rouiller I, Tweten RK, Wilson-Kubalek EM. Prepore to pore transition of a cholesterol-dependent cytolysin visualized by electron microscopy. J Struct Biol. 2005 Apr; 150(1):100-8. PMID: 15797734.
      View in: PubMed
    44. Zheng Z, Tweten RK, Mensa-Wilmot K. Intracellular glycosylphosphatidylinositols accumulate on endosomes: toxicity of alpha-toxin to Leishmania major. Eukaryot Cell. 2005 Mar; 4(3):556-66. PMID: 15755918; PMCID: PMC1087796.
    45. Polekhina G, Giddings KS, Tweten RK, Parker MW. Insights into the action of the superfamily of cholesterol-dependent cytolysins from studies of intermedilysin. Proc Natl Acad Sci U S A. 2005 Jan 18; 102(3):600-5. PMID: 15637162; PMCID: PMC545513.
    46. Giddings KS, Zhao J, Sims PJ, Tweten RK. Human CD59 is a receptor for the cholesterol-dependent cytolysin intermedilysin. Nat Struct Mol Biol. 2004 Dec; 11(12):1173-8. PMID: 15543155.
      View in: PubMed
    47. Czajkowsky DM, Hotze EM, Shao Z, Tweten RK. Vertical collapse of a cytolysin prepore moves its transmembrane beta-hairpins to the membrane. EMBO J. 2004 Aug 18; 23(16):3206-15. PMID: 15297878; PMCID: PMC514522.
    48. Zheng Z, Butler KD, Tweten RK, Mensa-Wilmot K. Endosomes, glycosomes, and glycosylphosphatidylinositol catabolism in Leishmania major. J Biol Chem. 2004 Oct 01; 279(40):42106-13. PMID: 15254033.
      View in: PubMed
    49. Ramachandran R, Tweten RK, Johnson AE. Membrane-dependent conformational changes initiate cholesterol-dependent cytolysin oligomerization and intersubunit beta-strand alignment. Nat Struct Mol Biol. 2004 Aug; 11(8):697-705. PMID: 15235590.
      View in: PubMed
    50. Polekhina G, Giddings KS, Tweten RK, Parker MW. Crystallization and preliminary X-ray analysis of the human-specific toxin intermedilysin. Acta Crystallogr D Biol Crystallogr. 2004 Feb; 60(Pt 2):347-9. PMID: 14747721.
      View in: PubMed
    51. Melton JA, Parker MW, Rossjohn J, Buckley JT, Tweten RK. The identification and structure of the membrane-spanning domain of the Clostridium septicum alpha toxin. J Biol Chem. 2004 Apr 02; 279(14):14315-22. PMID: 14715670.
      View in: PubMed
    52. Mourez M, Yan M, Lacy DB, Dillon L, Bentsen L, Marpoe A, Maurin C, Hotze E, Wigelsworth D, Pimental RA, Ballard JD, Collier RJ, Tweten RK. Mapping dominant-negative mutations of anthrax protective antigen by scanning mutagenesis. Proc Natl Acad Sci U S A. 2003 Nov 25; 100(24):13803-8. PMID: 14623961; PMCID: PMC283502.
    53. Giddings KS, Johnson AE, Tweten RK. Redefining cholesterol's role in the mechanism of the cholesterol-dependent cytolysins. Proc Natl Acad Sci U S A. 2003 Sep 30; 100(20):11315-20. PMID: 14500900; PMCID: PMC208754.
    54. Heuck AP, Tweten RK, Johnson AE. Assembly and topography of the prepore complex in cholesterol-dependent cytolysins. J Biol Chem. 2003 Aug 15; 278(33):31218-25. PMID: 12777381.
      View in: PubMed
    55. Ramachandran R, Heuck AP, Tweten RK, Johnson AE. Structural insights into the membrane-anchoring mechanism of a cholesterol-dependent cytolysin. Nat Struct Biol. 2002 Nov; 9(11):823-7. PMID: 12368903.
      View in: PubMed
    56. Wichroski MJ, Melton JA, Donahue CG, Tweten RK, Ward GE. Clostridium septicum alpha-toxin is active against the parasitic protozoan Toxoplasma gondii and targets members of the SAG family of glycosylphosphatidylinositol-anchored surface proteins. Infect Immun. 2002 Aug; 70(8):4353-61. PMID: 12117945; PMCID: PMC128134.
    57. Hotze EM, Heuck AP, Czajkowsky DM, Shao Z, Johnson AE, Tweten RK. Monomer-monomer interactions drive the prepore to pore conversion of a beta-barrel-forming cholesterol-dependent cytolysin. J Biol Chem. 2002 Mar 29; 277(13):11597-605. PMID: 11799121.
      View in: PubMed
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