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Influence of the in vitro culture period on the in vivo performance of cell/titanium bone tissue-engineered constructs using a rat cranial critical size defect model.
Mineralized matrix deposition by marrow stromal osteoblasts in 3D perfusion culture increases with increasing fluid shear forces.
In vitro generated extracellular matrix and fluid shear stress synergistically enhance 3D osteoblastic differentiation.
Effect of bone extracellular matrix synthesized in vitro on the osteoblastic differentiation of marrow stromal cells.
Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner.
Effect of fibronectin- and collagen I-coated titanium fiber mesh on proliferation and differentiation of osteogenic cells.
Flow perfusion culture of marrow stromal osteoblasts in titanium fiber mesh.