| Item Type | Name |
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Concept
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Mutagenesis, Site-Directed
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Concept
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Mutagenesis, Insertional
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Academic Article
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Kinetic analysis of YPD1-dependent phosphotransfer reactions in the yeast osmoregulatory phosphorelay system.
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Academic Article
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Role of the S128, H186, and N187 triad in substrate binding and decarboxylation in the sheep liver 6-phosphogluconate dehydrogenase reaction.
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Academic Article
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Proper positioning of the nicotinamide ring is crucial for the Ascaris suum malic enzyme reaction.
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Academic Article
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Effect of mutation of lysine-120, located at the entry to the active site of O-acetylserine sulfhydrylase-A from Salmonella typhimurium.
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Academic Article
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Glutamates 78 and 122 in the active site of saccharopine dehydrogenase contribute to reactant binding and modulate the basicity of the acid-base catalysts.
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Academic Article
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The multifaceted pyridoxal 5'-phosphate-dependent O-acetylserine sulfhydrylase.
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Academic Article
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Evidence in support of lysine 77 and histidine 96 as acid-base catalytic residues in saccharopine dehydrogenase from Saccharomyces cerevisiae.
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Academic Article
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Probing the chemical mechanism of saccharopine reductase from Saccharomyces cerevisiae using site-directed mutagenesis.
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Academic Article
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Mapping the active site topography of the NAD-malic enzyme via alanine-scanning site-directed mutagenesis.
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Academic Article
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Lysine 183 is the general base in the 6-phosphogluconate dehydrogenase-catalyzed reaction.
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Academic Article
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Alpha,beta-elimination reaction of O-acetylserine sulfhydrylase. Is the pyridine ring required?
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Academic Article
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Role of methionine-13 in the catalytic mechanism of 6-phosphogluconate dehydrogenase from sheep liver.
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Academic Article
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Site-directed mutagenesis as a probe of the acid-base catalytic mechanism of homoisocitrate dehydrogenase from Saccharomyces cerevisiae.
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Academic Article
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A change in the internal aldimine lysine (K42) in O-acetylserine sulfhydrylase to alanine indicates its importance in transimination and as a general base catalyst.
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Academic Article
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Cloning, expression, purification, and characterization of the 6-phosphogluconate dehydrogenase from sheep liver.
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Academic Article
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Cysteine 42 is important for maintaining an integral active site for O-acetylserine sulfhydrylase resulting in the stabilization of the alpha-aminoacrylate intermediate.
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Academic Article
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Expression and stereochemical and isotope effect studies of active 4-oxalocrotonate decarboxylase.
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Academic Article
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Supporting role of lysine 13 and glutamate 16 in the acid-base mechanism of saccharopine dehydrogenase from Saccharomyces cerevisiae.
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Academic Article
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Kinetic and chemical mechanisms of the sheep liver 6-phosphogluconate dehydrogenase.
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Academic Article
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Glutamate 190 is a general acid catalyst in the 6-phosphogluconate-dehydrogenase-catalyzed reaction.
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Academic Article
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Reaction mechanism of fructose-2,6-bisphosphatase. A mutation of nucleophilic catalyst, histidine 256, induces an alteration in the reaction pathway.
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Academic Article
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Lysine 199 is the general acid in the NAD-malic enzyme reaction.
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Academic Article
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Characterization of the S272A,D site-directed mutations of O-acetylserine sulfhydrylase: involvement of the pyridine ring in the alpha,beta-elimination reaction.
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Academic Article
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Exploring O-acetylserine sulfhydrylase-B isoenzyme from Salmonella typhimurium by fluorescence spectroscopy.
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Academic Article
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The oxidation state of active site thiols determines activity of saccharopine dehydrogenase at low pH.
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Academic Article
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Chemical mechanism of the fructose-6-phosphate,2-kinase reaction from the pH dependence of kinetic parameters of site-directed mutants of active site basic residues.
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Grant
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STRUCTURE AND MECHANISM OF NAD-MALIC ENZYME
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Grant
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Lysine Biosynthesis in Yeast
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Grant
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STRUCTURE/MECHANISM OF 6-PHOSPHOGLUCONATE DEHYDROGENASE
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