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Paul Cook to Mutagenesis, Site-Directed

This is a "connection" page, showing publications Paul Cook has written about Mutagenesis, Site-Directed.
Connection Strength

1.878
  1. Probing the chemical mechanism of saccharopine reductase from Saccharomyces cerevisiae using site-directed mutagenesis. Arch Biochem Biophys. 2015 Oct 15; 584:98-106.
    View in: PubMed
    Score: 0.498
  2. Characterization of the S272A,D site-directed mutations of O-acetylserine sulfhydrylase: involvement of the pyridine ring in the alpha,beta-elimination reaction. Biochemistry. 2003 Jan 14; 42(1):106-13.
    View in: PubMed
    Score: 0.208
  3. Supporting role of lysine 13 and glutamate 16 in the acid-base mechanism of saccharopine dehydrogenase from Saccharomyces cerevisiae. Arch Biochem Biophys. 2012 Jun 01; 522(1):57-61.
    View in: PubMed
    Score: 0.098
  4. The oxidation state of active site thiols determines activity of saccharopine dehydrogenase at low pH. Arch Biochem Biophys. 2011 Sep 15; 513(2):71-80.
    View in: PubMed
    Score: 0.094
  5. The multifaceted pyridoxal 5'-phosphate-dependent O-acetylserine sulfhydrylase. Biochim Biophys Acta. 2011 Nov; 1814(11):1497-510.
    View in: PubMed
    Score: 0.092
  6. Glutamates 78 and 122 in the active site of saccharopine dehydrogenase contribute to reactant binding and modulate the basicity of the acid-base catalysts. J Biol Chem. 2010 Jul 02; 285(27):20756-68.
    View in: PubMed
    Score: 0.086
  7. Site-directed mutagenesis as a probe of the acid-base catalytic mechanism of homoisocitrate dehydrogenase from Saccharomyces cerevisiae. Biochemistry. 2009 Aug 04; 48(30):7305-12.
    View in: PubMed
    Score: 0.082
  8. Proper positioning of the nicotinamide ring is crucial for the Ascaris suum malic enzyme reaction. Biochemistry. 2008 Feb 26; 47(8):2539-46.
    View in: PubMed
    Score: 0.074
  9. Effect of mutation of lysine-120, located at the entry to the active site of O-acetylserine sulfhydrylase-A from Salmonella typhimurium. Biochim Biophys Acta. 2008 Apr; 1784(4):629-37.
    View in: PubMed
    Score: 0.073
  10. Role of the S128, H186, and N187 triad in substrate binding and decarboxylation in the sheep liver 6-phosphogluconate dehydrogenase reaction. Biochemistry. 2006 Oct 24; 45(42):12680-6.
    View in: PubMed
    Score: 0.067
  11. Role of methionine-13 in the catalytic mechanism of 6-phosphogluconate dehydrogenase from sheep liver. Biochemistry. 2005 Feb 22; 44(7):2432-40.
    View in: PubMed
    Score: 0.060
  12. Alpha,beta-elimination reaction of O-acetylserine sulfhydrylase. Is the pyridine ring required? Biochim Biophys Acta. 2003 Apr 11; 1647(1-2):66-9.
    View in: PubMed
    Score: 0.053
  13. Lysine 199 is the general acid in the NAD-malic enzyme reaction. Biochemistry. 2000 Oct 03; 39(39):11955-60.
    View in: PubMed
    Score: 0.044
  14. Expression and stereochemical and isotope effect studies of active 4-oxalocrotonate decarboxylase. Biochemistry. 2000 Feb 01; 39(4):718-26.
    View in: PubMed
    Score: 0.042
  15. Lysine 183 is the general base in the 6-phosphogluconate dehydrogenase-catalyzed reaction. Biochemistry. 1999 Aug 31; 38(35):11231-8.
    View in: PubMed
    Score: 0.041
  16. Mapping the active site topography of the NAD-malic enzyme via alanine-scanning site-directed mutagenesis. Biochemistry. 1999 Aug 10; 38(32):10527-32.
    View in: PubMed
    Score: 0.041
  17. Glutamate 190 is a general acid catalyst in the 6-phosphogluconate-dehydrogenase-catalyzed reaction. Biochemistry. 1998 Nov 10; 37(45):15691-7.
    View in: PubMed
    Score: 0.039
  18. Cloning, expression, purification, and characterization of the 6-phosphogluconate dehydrogenase from sheep liver. Protein Expr Purif. 1998 Jul; 13(2):251-8.
    View in: PubMed
    Score: 0.038
  19. Kinetic and chemical mechanisms of the sheep liver 6-phosphogluconate dehydrogenase. Arch Biochem Biophys. 1996 Dec 15; 336(2):215-23.
    View in: PubMed
    Score: 0.034
  20. A change in the internal aldimine lysine (K42) in O-acetylserine sulfhydrylase to alanine indicates its importance in transimination and as a general base catalyst. Biochemistry. 1996 Oct 15; 35(41):13485-93.
    View in: PubMed
    Score: 0.034
  21. Evidence in support of lysine 77 and histidine 96 as acid-base catalytic residues in saccharopine dehydrogenase from Saccharomyces cerevisiae. Biochemistry. 2012 Jan 31; 51(4):857-66.
    View in: PubMed
    Score: 0.024
  22. Exploring O-acetylserine sulfhydrylase-B isoenzyme from Salmonella typhimurium by fluorescence spectroscopy. Arch Biochem Biophys. 2011 Jan 15; 505(2):178-85.
    View in: PubMed
    Score: 0.022
  23. Kinetic analysis of YPD1-dependent phosphotransfer reactions in the yeast osmoregulatory phosphorelay system. Biochemistry. 2005 Jan 11; 44(1):377-86.
    View in: PubMed
    Score: 0.015
  24. Reaction mechanism of fructose-2,6-bisphosphatase. A mutation of nucleophilic catalyst, histidine 256, induces an alteration in the reaction pathway. J Biol Chem. 1999 Jan 22; 274(4):2166-75.
    View in: PubMed
    Score: 0.010
  25. Chemical mechanism of the fructose-6-phosphate,2-kinase reaction from the pH dependence of kinetic parameters of site-directed mutants of active site basic residues. Biochemistry. 1997 Jul 22; 36(29):8775-84.
    View in: PubMed
    Score: 0.009
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