Coordinated Actions of Cas9 HNH and RuvC Nuclease Domains Are Regulated by the Bridge Helix and the Target DNA Sequence.

Coordinated Actions of Cas9 HNH and RuvC Nuclease Domains Are Regulated by the Bridge Helix and the Target DNA Sequence. Biochemistry. 2021 12 14; 60(49):3783-3800.

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subject areas

Binding Sites
Clustered Regularly Interspaced Short Palindromic Repeats
CRISPR-Cas Systems
Crystallography, X-Ray
DNA
DNA Cleavage
Gene Expression
Kinetics
Molecular Dynamics Simulation
Mutation
Protein Binding
Protein Conformation, alpha-Helical
Protein Conformation, beta-Strand
Protein Domains
Protein Interaction Domains and Motifs
RNA
RNA, Guide
Streptococcus pyogenes
Substrate Specificity
Thermodynamics

authors with profiles

Rakhi Rajan