Two-Hybrid System Techniques
                             
                            
                            
                                
                            
                            
                                
                            
                            
                            
                                
                                    
                                            
	"Two-Hybrid System Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, 
	MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure, 
	which enables searching at various levels of specificity.
	
	
		
			
			
				Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
    
			
			
				
				
					
						| Descriptor ID | D020798 | 
					
						| MeSH Number(s) | E05.393.220.870 E05.601.690.650 E05.601.870 | 
					
						| Concept/Terms | Two-Hybrid System TechniquesTwo-Hybrid System TechniquesTechnique, Two-Hybrid SystemTechniques, Two-Hybrid SystemTwo Hybrid System TechniquesTwo-Hybrid System TechniqueTwo-Hybrid MethodMethod, Two-HybridMethods, Two-HybridTwo Hybrid MethodTwo-Hybrid MethodsTwo-Hybrid System TechnicsTechnic, Two-Hybrid SystemTechnics, Two-Hybrid SystemTwo Hybrid System TechnicsTwo-Hybrid System TechnicTwo-Hybrid AssayAssay, Two-HybridAssays, Two-HybridTwo Hybrid AssayTwo-Hybrid Assays
 One-Hybrid System TechniquesOne-Hybrid System TechniquesOne Hybrid System TechniquesOne-Hybrid System TechniqueTechnique, One-Hybrid SystemTechniques, One-Hybrid SystemOne-Hybrid System TechnicsOne Hybrid System TechnicsOne-Hybrid System TechnicTechnic, One-Hybrid SystemTechnics, One-Hybrid System
 Yeast Two-Hybrid System TechniquesYeast Two-Hybrid System TechniquesYeast Two Hybrid System TechniquesYeast Two-Hybrid SystemSystem, Yeast Two-HybridSystems, Yeast Two-HybridTwo-Hybrid System, YeastTwo-Hybrid Systems, YeastYeast Two Hybrid SystemYeast Two-Hybrid SystemsYeast Two-Hybrid AssayAssay, Yeast Two-HybridAssays, Yeast Two-HybridTwo-Hybrid Assay, YeastTwo-Hybrid Assays, YeastYeast Two Hybrid AssayYeast Two-Hybrid Assays
 Three-Hybrid System TechniquesThree-Hybrid System TechniquesTechnique, Three-Hybrid SystemTechniques, Three-Hybrid SystemThree Hybrid System TechniquesThree-Hybrid System TechniqueTri-Hybrid System TechniquesTechnique, Tri-Hybrid SystemTechniques, Tri-Hybrid SystemTri Hybrid System TechniquesTri-Hybrid System TechniqueThree-Hybrid System TechnicsTechnic, Three-Hybrid SystemTechnics, Three-Hybrid SystemThree Hybrid System TechnicsThree-Hybrid System Technic
 Yeast Three-Hybrid System TechniquesYeast Three-Hybrid System TechniquesYeast Three Hybrid System TechniquesYeast Three-Hybrid SystemSystem, Yeast Three-HybridSystems, Yeast Three-HybridThree-Hybrid System, YeastThree-Hybrid Systems, YeastYeast Three Hybrid SystemYeast Three-Hybrid SystemsYeast Three-Hybrid AssayAssay, Yeast Three-HybridAssays, Yeast Three-HybridThree-Hybrid Assay, YeastThree-Hybrid Assays, YeastYeast Three Hybrid AssayYeast Three-Hybrid Assays
 n-Hybrid System Techniquesn-Hybrid System TechniquesSystem Technique, n-HybridSystem Techniques, n-HybridTechnique, n-Hybrid SystemTechniques, n-Hybrid Systemn Hybrid System Techniquesn-Hybrid System Technique
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				Below are MeSH descriptors whose meaning is more general than "Two-Hybrid System Techniques".
				
			 
			
			
				Below are MeSH descriptors whose meaning is more specific than "Two-Hybrid System Techniques".
				
			 
		 
	 
 
                                        
                                            
	
	
		
			
			
					
				This graph shows the total number of publications written about "Two-Hybrid System Techniques" by people in this website by year, and whether "Two-Hybrid System Techniques" was a major or minor topic of these publications. 
				
					 
                    To see the data from this visualization as text, 
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		            | Year | Major Topic | Minor Topic | Total | 
|---|
| 2000 | 0 | 1 | 1 | 
| 2002 | 0 | 1 | 1 | 
| 2003 | 0 | 4 | 4 | 
| 2004 | 0 | 2 | 2 | 
| 2005 | 0 | 3 | 3 | 
| 2006 | 0 | 2 | 2 | 
| 2007 | 0 | 1 | 1 | 
| 2008 | 0 | 2 | 2 | 
| 2009 | 0 | 1 | 1 | 
| 2011 | 1 | 1 | 2 | 
| 2013 | 0 | 1 | 1 | 
| 2014 | 0 | 1 | 1 | 
| 2018 | 0 | 1 | 1 | 
| 2020 | 0 | 1 | 1 | 
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				Below are the most recent publications written about "Two-Hybrid System Techniques" by people in Profiles.
						
					
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								Proapoptotic Mitochondrial Carrier Homolog Protein PSAP Mediates Death Receptor 6 Induced Apoptosis. J Alzheimers Dis. 2020; 74(4):1097-1106. 
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								Redundant and Nonredundant Functions of Akt Isoforms in the Retina. Adv Exp Med Biol. 2018; 1074:585-591. 
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								Selective inhibition of rDNA transcription by a small-molecule peptide that targets the interface between RNA polymerase I and Rrn3. Mol Cancer Res. 2014 Nov; 12(11):1586-96. 
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								NUCLEAR FACTOR Y transcription factors have both opposing and additive roles in ABA-mediated seed germination. PLoS One. 2013; 8(3):e59481. 
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								Construction of high quality Gateway™ entry libraries and their application to yeast two-hybrid for the monocot model plant Brachypodium distachyon. BMC Biotechnol. 2011 May 19; 11:53. 
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								Towards establishment of a rice stress response interactome. PLoS Genet. 2011 Apr; 7(4):e1002020. 
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								Sclerostin binds and regulates the activity of cysteine-rich protein 61. Biochem Biophys Res Commun. 2010 Jan 29; 392(1):36-40. 
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								A rice kinase-protein interaction map. Plant Physiol. 2009 Mar; 149(3):1478-92. 
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								Identification and functional characterization of an N-terminal oligomerization domain for polycystin-2. J Biol Chem. 2008 Oct 17; 283(42):28471-9. 
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								A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas. J Cell Biol. 2007 Mar 12; 176(6):819-29.